Objective  To establish the HPLC fingerprint of the co-decoction of Serissa japonica and Humulus scandens (hereinafter referred to as “Xuelyu”) and conduct multi-component quantitative analysis to evaluate the quality of “Xuelyu” drug pair.

Methods  Agilent EC-C₁₈ column (4.6 mm × 150 mm, 4 μm) was used for detection. Methanol-0.1% aqueous phosphoric acid solution was used as mobile phase and eluted gradientally; the flow rates were 1.0 mL·min1 for 0-30 min and 0.6 mL·min^-1 for 30-65 min; the detection wavelengths were 350 nm and 240 nm; the column temperature was 30 °C. The "Similarity Evaluation system Software for Traditonal Chinese Medicine Fingerprint (2012.1) Edition" co-decoction solution of “Xuelyu” drug  pair was evaluated by similarity evaluation, The four index components were quantitatively analyzed and compared before and after compatibility.

Results  In the study of characteristic fingerprintgraph, 12 common peaks were calibrated, with No.4 peak as the reference peak, 10 batches of "Xuelyu" drug pairs had good similarity between the common decoction solution and the common mode control graph, and the similarity was above 0.9; under the established chromatographic conditions, the components had a good linear relationship, and the average dosing recovery rate was between 98.7% and 99.6%. RSD values were all less than 3% (n=6). The contents of plantain glycolate, luteolin, rutin and cosmos in the 10 batches of "Xuelyu" drug pairs in the co-decoction ranges were 0.249 4-0.402 8 mg·g^-1, 0.109 1-0.238 9 mg·g^-1, 0.045 9-0.076 8 mg·g^-1, 0.091 4-0.173 7 mg·g^-1, respectively. After the combination of drug pairs, the contents of luteolin, rutin and cosmopolitan in the decoction were all decreased, and the decreasing rates were 296.40%, 47.86% and 25.02%, respectively, while the content of plantain glycolate was increased and the increasing rate was 8.81%.The content of cosmopolitan was significantly decreased (P<0.05), and the content of luteolin and rutin was significantly decreased (P<0.01).

Conclusion  The established HPLC fingerprint and content determination method of co-decoction solution have high sensitivity and strong specificity which can be used for the quality control of the drug pair and also laid the foundation for its compatibility mechanism and pharmacological research.

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