Objective  To analyze and identify the volatile constituents in different parts (flowers, stems and leaves) of Huai chrysanthemumin, and to lay a theoretical foundation for the comprehensive utilization for it.

Methods  The volatile oil in different parts of Huai chrysanthemumin were extracted by hydrodistillation, respectively. Their constituents were analyzed by gas chromatography-mass spectrometry (GC-MS). The compounds were identified by library search and literature screening. The relative percentage of each compound was obtained by the area normalization method. The differences in their chemical compositions were analyzed by Venn diagram, principal component analysis (PCA) and cluster heat map analysis.

Results  A total of 62 volatile chemical components were identified from different parts of Huai chrysanthemumin, including monoterpenes, sesquiterpenes, and their derivatives, as well as a small amount of aliphatic compounds. 32, 42 and 40 volatile components were detected from the flowers, stems and flowers, respectively. Furthermore 17 volatile components were shared by three parts, whereas 5, 6 and 16 volatile components were unique to the flowers, stems and leaves, respectively. The results of stoichiometric analysis showed that both PCA and cluster heat map analysis could separate the flowers, stems and leaves, and their volatile components were different.

Conclusion  The types and contents of the volatile oil in the stems, leaves and flowers of Huai chrysanthemumin have certain variability, which provide a scientific basis for the further medicinal or industrial exploitation of different parts of Huai chrysanthemumin.

1.中国药典2020年版. 一部[S]. 2020: 233.

2.杨建宇, 刘冠军, 刘白云, 等. 中华中医药道地药材系列汇讲(13): 道地药材怀菊花的研究近况[J]. 现代医学与健康研究, 2020, 4(13): 98-101. [Yang JY, Liu GJ, Liu BY, et al. A series of lectures on traditional Chinese medicine of China genuine medicinal materials (13): the recent research status of genuine medicinal materials Huai chrysanthemum[J]. Modern Medicine and Health Research, 2020, 4(13): 98-101.] https://d.wanfangdata.com.cn/periodical/xdyxyjkyj202013046.

3.高素霞, 刘国彬, 王飞, 等. 药用菊花不同品种抗蚜性评价[J]. 中国植保导刊, 2023, 43(9): 60-64. [Gao SX, Liu GB, Wang F, et al. Evaluation of aphid resistance of different medicinal chrysanthemums varieties[J]. China Plant Protection, 2023, 43(9): 60-64.] DOI: 10.3969/j.issn.1672-6820.2023.09.010.

4.代震, 利顺欣, 陈随清, 等. 怀菊花种质资源品质评价[J]. 中国实验方剂学杂志, 2017, 23(6): 48-54. [Dai Z, Li SX, Chen SQ, et al. Quality evaluation on germplasm resources of Huai chrysanthemi Flos[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2017, 23(6): 48-54.] DOI: 10.13422/j.cnki.syfjx.2017060048.

5.袁一平, 孔李婷, 郭兆娟, 等. 怀菊花的历史源流分析及其现代技术标准体系初步设计[J]. 中国现代中药, 2018, 20(1): 117-121. [Yuan YP, Kong LT, Guo ZJ, et al. Analysis on the historical origin of Huai chrysanthemum and preliminary design of modern technical standard system[J]. Modern Chinese Medicine, 2018, 20(1): 117-121.] DOI: 10.13313/j.issn.1673-4890.20170607002.

6.余永亮, 许兰杰, 杨红旗, 等. 河南花类道地药材资源的区域分布及其产业现状分析研究[J]. 中国中药杂志, 2019, 44(5): 899-904. [Yu YL, Xu LJ, Yang HQ, et al. Research on regional distribution and industrial status analysis of genuine medicinal resources of flowers in Henan province[J]. China Journal of Chinese Materia Medica, 2019, 44(5): 899-904.] DOI: 10.19540/j.cnki.cjcmm.20181226.002.

7.周衡朴, 任敏霞, 管家齐, 等 .菊花化学成分、药理作用的研究进展及质量标志物预测分析[J]. 中草药, 2019, 50(19): 4785-4795. [Zhou HP, Ren MX, Guan JQ, et al. Research progress on chemical constituents and pharmacological effects of Chrysanthemum morifolium and predictive analysis on quality markers[J]. Chinese Traditional and Herbal Drugs, 2019, 50(19): 4785-4795.] DOI: 10.7501/j.issn.0253-2670.2019.19.030.

8.刘夏进, 李懿, 宿树兰, 等. 药用菊非药用部位的资源化利用现状与展望[J]. 中草药, 2020, 51(15): 4075-4081. [Liu XJ, Li Y, Su SL, et al. Status and prospects of resource utilization of non-medicinal parts of medicinal Chrysanthemum morifolium[J]. Chinese Traditional and Herbal Drugs, 2020, 51(15): 4075-4081.] DOI: 10.7501/j.issn.0253-2670.2020.15.028.

9.张月荣, 刘晓丽, 詹海杰, 等. 中药非药用部位在动物生产中的应用[J]. 饲料研究, 2023, 46(3): 148-153. [Zhang YR, Liu XL, Zhan HJ, et al. Application of non-medicinal parts of traditional Chinese medicine in animal production[J]. Feed Research, 2023, 46(3): 148-153.] DOI: 10.13557/j.cnki.issn1002-2813.2023.03.030.

10.张微, 李吉平, 刘洋, 等. 道地药材发展现状及创新驱动机制研究[J]. 齐齐哈尔医学院学报, 2023, 44(5): 479-482. [Zhang W, Li JP, Liu Y, et al. Research on development status and innovation driving mechanism of authentic medicinal materials[J]. Journal of Qiqihar Medical Uniwersity, 2023, 44(5): 479-482.] DOI: 10.3969/j.issn.1002-1256.2023.05.019.

11.海城英, 白秀云, 陈亨业, 等. 气质联用结合化学计量学对中国白菊特征挥发性成分的研究[J]. 化学试剂, 2021, 43(5): 584-589. [Hai CY, Bai XY, Chen HY, et al. Study on characteristic volatile components of Chinese White Chrysanthemum by GC-MS combined with chemometrics[J]. Chemical Reagent, 2021, 43(5): 584-589.] DOI: 10.13822/j.cnki.hxsj.2021008155.

12.丁云龙, 王浩东, 唐国玮, 等. 5种药食兼用菊花挥发性有机物的HS-GC-IMS与指纹图谱[J]. 食品与机械, 2022, 38(9): 52-58. [Ding YL, Wang HD, Tang GW, et al. HS-GC-IMS analysis and fingerprint study of volatile organic compounds in five species medicinal and edible Chrysanthemum morifolium Ramat [J]. Food & Machinery, 2022, 38(9): 52-58.] DOI: 10.13652/j.spjx.1003.5788. 2022.90145.

13.宋佳慧, 夏熠珣, 沈慧娟, 等. 8种饮用菊茶汤的感官风味与活性成分比较[J]. 食品工业科技, 2021, 42(12): 24-31. [Song JH, Xia YX, Shen HJ, et al. Comparison of the sensory quality and active ingredients of 8 kinds of Chrysanthemum tea infusion[J]. Science and Technology of Food Industry, 2021, 42(12): 24-31.] DOI:10.13386/j.issn1002-0306.2020090088.

14.Kuang CL, Luy D, Shen GH, et al. Chemical composition and antimicrobial activities of volatile oil extracted from Chrysanthemum morifolium Ramat[J]. J Food Sci  Technol, 2018, 55(7): 2786-2794. DOI: 10.1007/s13197-018-3203-1.

15.Sun QL, Hua S, Ye JH, et al. Flavonoids and volatiles in Chrysanthemum morifolium Ramat flower from Tongxiang County in China[J]. Afr J Biotechnol, 2010, 9 (25): 3817-3821. DOI: 10.5897/AJB2010.000-3252.

16.Wu YB, Yan ZM, Zhao JJ, et al. Headspace solid-phase microextraction and gas chromatography-mass spectrometry of volatile components of Chrysanthemum morifolium Ramat[J]. Trop J Pharm Res, 2016, 15(10): 2241-2244. DOI: 10.4314/tjpr.v15i10.24.

17.Yang M, Tian X, Zhang M, et al. A holistic comparison of flavor signature and chemical profile in different harvesting periods of Chrysanthemum morifolium Ramat. based on metabolomics combined with bioinformatics and molecular docking strategy[J]. RSC Adv, 2022, 12(54): 34971-34989. DOI: 10.1039/d2ra05698d.

18.杨丹. 传统药用菊花品种差异比较及质量标准研究[D]. 北京: 中国中医科学院, 2022.

19.Zeng M, Li M, Chen Y, et al. A new bisepoxylignan dendranlignan A isolated from Chrysanthemum flower inhibits the production of inflammatory mediators via the TLR4 pathway in LPS-induced H9c2 cardiomyocytes[J]. Arch Biochem Biophys, 2020, 690: 108506. DOI: 10.1016/j.abb.2020.108506.

20.Li Meng, Zeng Mengnan, Zhang Jingke, et al. Anti-inflammatory Dendranacetylene A, a new polyacetylene glucoside from the flower of Chrysanthemum morifolium Ramat[J]. Nat Prod Res, 2021, 35(24): 5692-5698. DOI: 10.1080/14786419.2020.1825425.

21.Zhang B, Li M, Shi J, et al. Six sesquiterpenoids from the stems and leaves of Chrysanthemum morifolium Ramat and their anti-asthma activities[J]. Fitoterapia, 2023, 171: 105633, DOI: 10.1016/j.fitote.2023.105633.

22.Chen WJ, Zeng, MN, Li, M, et al. Four new sesquiterpenoids from Dendranthema morifolium (Ramat.) kitam flowers[J]. Phytochem Lett, 2018, 23, 52-56. DOI: 10.1016/j.phytol.2017.11.009.

23.中国药典2020年版. 四部[S]. 2020: 233.

24.刘晓薇, 张飞, 陈随清, 等. 不同商品规格怀菊花的质量特征分析[J]. 中华中医药杂志, 2018, 33(6): 2650-2655. [Liu XW, Zhang F, Chen SQ, et al. Analysis on quality characteristics in different commercial grades of Huai chrysanthemi Flos[J]. Chinese Journal of Traditional Chinese Medicine, 2018, 33(6): 2650-2655.] DOI: CNKI:SUN:BXYY.0.2018-06-118.

25.魏婧, 唐丽杰, 娄晓月, 等. 荆条不同部位挥发油成分的GC-MS分析[J]. 食品工业科技, 2022, 43(12): 310-316. [Wei J, Tang LJ, Lou XY, et al. GC-MS analysis of constituents of volatile oil in different parts of Vitex negundo var. Heterophylla[J]. Science and Technology of Food Industry, 2022, 43(12): 310-316.] DOI: 10.13386/j.issn1002-0306.2021100145.

26.田震, 徐亚元, 李大婧, 等. 基于SPME-GC-MS分析不同干燥方式对香葱挥发油成分的影响[J]. 食品工业科技, 2021, 42(4): 232-244. [Tian Z, Xu YY, Li DJ, et al. Analysis of volatile components in dried chives by SPME-GC-MS[J]. Science and Technology of Food Industry, 2021, 42(4): 232-244.] DOI: 10.13386/j.issn1002-0306. 2020040349.

27.郑文迪, 关倩倩, 刘长根, 等. 基于 GC-MS 法对比广西地区酸菜和酸笋风味的差异[J]. 食品与发酵工业，2020, 46(4): 253-257. [Zheng WD, Guan QQ, Liu CG, et al. Comparison of flavors of Suancai and Suansun in Guangxi based on GC-MS [J]. Food and Fermentation Industries, 2020, 46(4): 253-257.] DOI: 10.13995/j.cnki.11-1802/ts.022474.

28.谢占芳. 八种菊花挥发性成分及其抑菌活性研究[D]. 河南开封: 河南大学, 2016.

29.吴仁海, 刘红彦, 尹新明, 等. 菊花茎叶挥发油的GC-MS分析[J]. 河南中医学院学报, 2008, 23(5): 26-27. [Wu RH, Liu HY, Yin XM, et al. Study on essential oil of Chrysanthemum's tender stems and leaves by GC-MS[J]. Journal of Henan University of Chinese Medicine, 2008, 23(5): 26-27.] DOI: 10.16368/j.issn.1674-8999. 2008.05.043.

30.陈筱溪. 芳香型菊花新品种选育及精油挥发性成分分析[D]. 成都: 四川农业大学, 2021.

31.田红女,张晓冬,王海燕. HPLC多组分定量结合化学计量学及灰色关联度的葆宫止血颗粒质量评价 [J].中国现代中药, 2024-01-09. [Tian HN, Zhang XD, Wang HY. Quality evaluation of Baogong Zhixue granule by HPLC multi-component quantitative combined with chemometrics and grey relational analysis[J]. Modern Chinese Medicine, 2024-01-09.] DOI: org/10.13313/j.issn.1673-4890.20230614001.

32.于洋,李军,李宝国. 化学计量学在中药质量控制研究中的应用[J]. 中成药, 2018, 40(5): 1139-1142. [Yu Y, Li J, Li BG. Application of chemometrics in the study of Chinese medicine quality control[J]. Chinese Traditional Patent Medicine, 2018, 40(5): 1139-1142.] DOI: 10.3969/j.issn.1001-1528.2018.05.028.