Chemical constituent identification of Zhuangxi-6 powders based on HPLC-Q-Exactive-MS

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Author: ZHANG Hao ¹ ZHANG Qian ²  LI Jun ²

Affiliation: 1. Department of Gastroenterology, Bayannaoer Hospital, Bayannaoer 015000, Inner Mongolia, China 2. College of Pharmacy, Inner Mongolia Medical University, Hohhot 010110, China

Keywords: Zhuangxi-6 powders Dyspepsia HPLC-Q-Exactive-MS Qualitative analysis Chemical constituents

DOI: 10.12173/j.issn.1008-049X.202401128

Reference: ZHANG Hao, ZHANG Qian, LI Jun.Chemical constituent identification of Zhuangxi-6 powders based on HPLC-Q-Exactive-MS[J].Zhongguo Yaoshi Zazhi,2024, 27(2):181-191.DOI: 10.12173/j.issn.1008-049X.202401128.[Article in Chinese]  Copied

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Abstract

Objective To quickly analyze and identify the chemical constituents in Zhuangxi-6 powders by HPLC-Q-Exactive-MS.

Methods The separation was performed on Shim-pack GIST C₁₈ column (150 mm×4.6 mm, 5 μm) with the mobile phase of methanol- 0.1% formic acid aqueous solution (gradient elution). The flow rate was 0.35 mL/min, column temperature was 35℃, and the volume of sample injection was 10 μL. Electrospray ionization was applied for scanning under positive and negative ion modes with the scanning range of m/ z 110-1 200.

Results Combining mass spectrometry data and literature information, a total of 65 components were identified in Zhuangxi-6 powders, including 16 alkaloids, 11 flavonoids, 14 organic acids, 6 chromones, 7 sesquiterpenoids, 4 fatty acids, 3 amino acids, 2 tannins, 1 phenolic acids, and 1 phenylpropanoid.

Conclusion HPLC-Q-Exactive-MS method can quickly identify the chemical components of Zhuangxi-6 powders, laying a foundation for the further research on the pharmacodynamics substances of Zhuangxi-6 powders.

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