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Home Articles Vol 27,2024 No.6 Detail

HPLC fingerprint and three main components determination of Modified Zengye Decoction

Published on Jul. 01, 2024Total Views: 97 times Total Downloads: 41 times Download Mobile

Author: LIU Shiqi 1, 3 WU Chuang 1, 2 ZHU Zhimin 1, 2 XU Feng 1, 2 CHANG Yaowen 1, 2 ZHANG Haiyan 1, 2 GOU Xiaojun 1, 3

Affiliation: 1. Department of Proctology, Baoshan Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201999, China 2. Department of Proctology, Baoshan District Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai 201999, China 3. Central Laboratory, Baoshan District Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai 201999, China

Keywords: Modified Zengye Decoction Ferulic acid Aurantiamarin Harpagoside High performance liquid chromatography Fingerprint Content determination Quality control

DOI: 10.12173/j.issn.1008-049X.202311200

Reference: LIU Shiqi, WU Chuang, ZHU Zhimin, XU Feng, CHANG Yaowen, ZHANG Haiyan,GOU Xiaojun.HPLC fingerprint and three main components determination of Modified Zengye Decoction[J].Zhongguo Yaoshi Zazhi,2024, 24(6):928-936.DOI: 10.12173/j.issn.1008-049X.202311200.[Article in Chinese]

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Abstract

Objective  To establish a method for HPLC fingerprint analysis and determine three main components of Modified Zengye Decoction.

Methods  The chromatographic column was Shimadzu WondaSil C18 column (250 mm×4.6 mm, 5 μm), the mobile phase was acetonitrile-0.3% aqueous phosphoric acid with a gradient elution procedure, the volume flow rate was 1.0 mL/min, the detection wavelengths were 265, 203, 310 and 290 nm, the column temperature was 25 °C, and the injection volume was 20 µL. The HPLC fingerprints of the 10 batches of Modified Zengye Decoction were established, and the similarity analysis was performed by using the similarity evaluation system of chromatographic fingerprint of traditional Chinese medicine (version 2012A). The common peaks were identified and assigned, and the contents of the three main components were quantitatively determined.

Results  There were 17 common peaks in the fingerprints of 10 batches of Modified Zengye Decoction with similarities ranging from 0.872-0.989. The fingerprints recognized peak 9, 14 and 17 as ferulic acid, aurantiamarin and harpagoside, respectively. The contents of ferulic acid, aurantiamarin and harpagoside were 0.067 3-0.174  8, 0.498 8-1.522 7, 0.270 9-0.802 4 mg/g, and the transfer rate were 30.74%-55.63%, 11.77%-35.94%, 23.15%-68.56%, respectively.

Conclusion  The established HPLC fingerprint analysis method combined with main components quantitative analysis method can be used for the quality analysis and control of Modified Zengye Decoction with simple analysis method and reliable results.

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