The clinical value of UPLC-MS/MS method for the determination of plasma and tissue isoniazid concentration in lymph node tuberculosis patients

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Author: Yu ZHANG ¹ Xiao-Yu LIU ² Feng XU ² Huan ZHANG ¹ Qiao-Yan DING ¹ Li-Hua MA ¹ Si-Si LI ¹  Ming ZHOU ¹

Affiliation: 1. Department of Pharmacy, Wuhan Pulmonary Hospital, Wuhan 430030, China 2. Department of Surgical, Wuhan Pulmonary Hospital, Wuhan 430030, China

Keywords: Lymph node tuberculosis Isoniazid Ultra performance liquid chromatography-mass spectrum/mass spectrum

DOI: 10.12173/j.issn.1008-049X.202303215

Reference: Yu ZHANG, Xiao-Yu LIU, Feng XU, Huan ZHANG, Qiao-Yan DING, Li-Hua MA, Si-Si LI, Ming ZHOU.The clinical value of UPLC-MS/MS method for the determination of plasma and tissue isoniazid concentration in lymph node tuberculosis patients[J].Zhongguo Yaoshi Zazhi,2023, 26(11):311-318.DOI: 10.12173/j.issn.1008-049X.202303215.[Article in Chinese]  Copied

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Abstract

Objective To investigate the correlation between isoniazid concentration in plasma and lymph node tissue of patients with lymph node tuberculosis, and to explore its clinical value.

Methods The basic information of patients with lymph node tuberculosis in our hospital and venous blood samples and neck lymph node tissue samples at different time points were collected. UPLC-MS/MS method was established and isoniazid concentration in plasma and neck lymph node tissue samples was quantitatively detected, and the correlation between isoniazid concentration in plasma and lymph node tissue was analyzed.

Results The linear range of isoniazid blood concentration and lymph node tissue concentration were 0.25-16 μg·mL^-1 (r=0.999 8) and 2-128 μg·g^-1 (r=0.998 8), respectively. The precision, accuracy, and matrix effect of each quality control sample met the requirements. Plasma isoniazid concentration and lymph node tissue isoniazid concentration were significantly correlated at 10 min (r_s= 0.501 1, P=0.001), 30 min (r_s= 0.402 8, P=0.005) and 60 min (r_s= 0.614 6, P=0.001) after intravenous infusion of isoniazid. The ratio of lymph node tissue isoniazid concentration to plasma isoniazid concentration was 1.46 (0.62, 3.55) mL·g^-1 at 10 min. At 30 min, the ratio was 5.25 (4.61, 11.61) mL·g^-1. At 60 min, the ratio was 6.62 (4.42, 10.78) mL·g^-1.

Conclusion The established UPLC-MS/MS method has good specificity, high sensitivity, accurac and precision. Monitoring plasma isoniazid concentration provides a reference for the rational use of isoniazid in patients with lymphatic tuberculosis.

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